2024 Thy1 gcamp6f

Thy1 gcamp6f

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August undefined, 2024

WebbUtilizing Miniature Fluorescence Microscopy to Image Hippocampal Place Cell Ensemble Function in Thy1.GCaMP6f Transgenic Mice. Curr Protoc Pharmacol Sep 2024 Other authors. Direct Imaging of Hippocampal Epileptiform Calcium Motifs Following Kainic Acid Administration in Freely Behaving Mice Front ... WebbThe laminar electrophysiological recordings during passive viewing (Fig. 2, A and B) were carried out in six Tg (Thy1-GCaMP6f)GP5.17Dkim mice (five males and one female) aged between 2 and 6 months. We implanted the mice with a head bar and one or two small screws over the cerebellum, which served as reference and ground and made a …

Resting-state hemodynamics are spatiotemporally coupled to

WebbIn a Thy1::GCaMP6f transgenic mouse, PSAM 4-GlyR was expressed in hippocampal pyramidal cells by injection of rAAV1-CamkII::PSAM 4-GlyR–IRES–EGFP . The field of view contained distinct domains of neurons densely and sparsely transduced with PSAM 4-GlyR–IRES–EGFP . WebbNational Center for Biotechnology Information python erosion

Thy1-GCaMP6 Transgenic Mice for Neuronal Population Imaging …

Webb17 juni 2024 · GCaMP6f, jGCaMP7f, jGCaMP7b ... Dana, H. et al. Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population … WebbArticle Snippet: Thy1-GCaMP6f mouse littermates (C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J, The Jackson Laboratory Stock No. 025393) ( ) were weaned at postnatal day 21 and housed together in temperature and humidity controlled facilities with 12-hour light/dark cycles and ad libitum water and food. Webb19 juni 2024 · GCaMP6f is among the most widely used genetically encoded calcium indicators for monitoring neuronal activity. Applications are at both the cellular and … python era5画风场

The essential role of recurrent processing for figure-ground

Webb11 okt. 2024 · Calcium imaging is commonly used to measure the neural activity of large groups of neurons in mice. Genetically encoded calcium indicators (GECIs) can be delivered for this purpose using non-invasive genetic methods. Compared to viral gene transfer, transgenic targeting of GECIs provides stable long-term expression and … Webb1 mars 2024 · To model physiological conditions of chronic stress on hippocampal function, transgenic mice expressing the genetically encoded calcium indicator … python era5Webb5 apr. 2024 · Qualitatively, imaging the cortex of a Thy1-GCaMP6f mouse 23, which expresses fluorescent reporters of calcium activity in excitatory neurons, with the mini … python eri suuri

"WebbMovie S1 shows recorded GCaMP6f data in the awake brain before and after hemodynamic correction, with concurrent Δ[HbT] (also shown as plots in Fig. S1). Fig. S2 shows data acquired on a Thy1-YFP mouse demonstrating expected strong hemodynamic cross talk, but only small variance attributable to intrinsic flavo- " - Thy1 gcamp6f

Thy1 gcamp6f

High-performance calcium sensors for imaging activity in ... - Nature

WebbGCaMP6f expression allowed for population-based calcium recording from cortical excitatory neurons, in addition to concurrently acquired hemodynamics. All mice were fitted with a transparent Plexiglas window to facilitate imaging. Webb17 juni 2024 · The widely used GCaMP6 sensors enable the detection of single action potentials under favorable conditions 4. They can be used to monitor the activity of large groups of neurons in vivo using...

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Webb1 apr. 2024 · Here, we follow glioma progression in awake Thy1-GCaMP6f mice using in vivo wide-field optical mapping to monitor alterations in both neuronal activity and functional hemodynamics. WebbAdult Thy1 Gcamp6f Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Webb23 feb. 2024 · Simultaneous electrophysiological and two-photon imaging recording of L2/3 excitatory neurons activity in GCaMP6f mice. a. Top. Experimental scheme. Bottom. Representative traces showing the same... Webb19 okt. 2024 · Transgenic mice expressing GCaMP6 driven by the thymus cell antigen 1 (Thy1) promotor were acquired from Jackson Laboratories (JAX Strain: C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim; stock: 024276)[17, 26]. In this model, GCaMP6 expression is widespread in the CNS (e.g. cortex, hippocampus, thalamus, and cerebellum) and …

WebbDiffusely infiltrating gliomas are known to cause alterations in cortical function, vascular disruption, and seizures. These neurological complication… WebbHere we present the development and characterization of transgenic mouse lines expressing GCaMP6s and GCaMP6f GECIs under the Thy1 promoter [20,21,22]. We …

Webb19 juni 2024 · GCaMP6f is among the most widely used genetically encoded calcium indicators for monitoring neuronal activity. Applications are at both the cellular and population levels. Here, we explore two important and under-explored issues.

WebbHere we present the development and characterization of transgenic mouse lines expressing GCaMP6s and GCaMP6f GECIs under the Thy1 promoter [20,21,22]. We characterize the brain-wide expression... python erpWebbThy1-GCaMP6f-WPRE (founder line GP5.12) improved SNR, faster kinetics, variable neuronal expression pattern: 028281: Dana et al., 2015: Thy1-GCaMP6f-WPRE (founder line GP5.17) improved SNR, faster kinetics, variable neuronal expression pattern. 025393. Dana et al., 2015 . Flies available from Bloomington Stock Center: python erosion skimageWebbneurons, as controlled by the Thy1 promoter. For these experiments, we used 3 Thy1-GCaMP6f male mice, with an average age of seven months, 25–28 g weight. 2.2. Surgery We performed a thinned skull preparation on the right hemisphere between bregma and lambda to create an optical window for GCaMP6f imaging. After applying the local … python errata_toolWebb13 sep. 2024 · (E and F) Setup for two-photon laser scanning microscopy (TPLSM) used for live imaging of mitochondria in individual RGC axons from Thy1-CFP-MitoS mice. (G and H) Representative kymographs of TPLSM-recorded mitochondrial movement show anterograde (magenta) and retrograde (green) transport in sham and ocular … python es bulk 批量更新Webb3 apr. 2024 · Therefore, we analyzed neural calcium imaging data from Thy1-GCaMP6f mice while either awake, asleep, anesthetized, during low and high bouts of motion, or before and after photothrombotic stroke. python errantWebb15 mars 2024 · To generate GCaMP6 transgenic mice, we utilized the previously described GCaMP6 fast and slow cDNA constructs (referred as GCaMP6F and GCaMP6S, respectively) and the well-characterized Thy1.2 promoter, which drives transgene expression in subsets of neurons (Fig. 1 A) (Chen et al., 2012; Feng et al., 2000).We … python esaWebbThe GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics. 024276 - GP5.5 Strain Details If you need help with your … python erp tutorial