Mtor western blot
Web14 apr. 2024 · (F–I) Western blot analysis showed the expression of P-AMPK/GAPDH, AMPK/GAPDH, P-mTOR/GAPDH and mTOR/GAPDH proteins. ( N , O ) The expression of NLRP3 (50× magnification) in BV2 microglia was … WebThe mechanistic target of rapamycin (mTOR) is an atypical serine/threonine kinase that is present in two distinct complexes. The first, mTOR complex 1 (mTORC1), is composed of mTOR, Raptor, GβL, and DEPTOR and is inhibited by rapamycin. It is a master growth regulator that senses and integrates diverse nutritional and environmental cues ...
Mtor western blot
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WebMtor-ZcKO GV oocytes were microinjected with Prc1 mRNA and matured in vitro for 18 h. Meiotic status was then analyzed by IF staining of chromosomes and spindles. (C, Upper) The Western blot gel image detecting PRC1 expression. (Lower) The graph shows the quantification of Western blot results
Web21 iul. 2024 · V5.Luciferase was used as control. After immunoprecipitation (IP) with anti-V5 antibody, the immobilized mTOR was detected by Western blot (WB) analysis using anti … WebThe mTOR Pathway Antibody Sampler Kit contains reagents to investigate the control of protein translation, cell growth, and proliferation through mTOR signaling within cells. …
Web28 mai 2013 · 分析测试百科 想检测hela细胞中mTOR 分子的表达和磷酸化情况，western blo实验做了3次，可是都没有目的条带，很是着急，还请各位帮我分析下啊～～磷酸化 … Web26 aug. 2015 · The blot with the mw higher than 250 is your mTOR and for those less than that it can't be mTOR it self. To avoid these bands don't use ingredient gels instead use …
WebWe used the immortalized UCC cell lines T24, RT4, J82, and UMUC3, which demonstrated baseline phosphorylation of mTOR and S6 by Western blot analysis. Cells were serum-starved for 24 hours and incubated with 1 pM to 1 μmol/L rapamycin for 48 hours. Control cells were incubated under parallel conditions with the solvent DMSO.
WebWestern blot analysis of NIH/3T3, using Phospho-mTOR-S2448 Rabbit mAb (AP1413) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes … is alcohol toxic to the bodyWeb26 feb. 2024 · Tissue samples obtained from treated/untreated diabetic mice and age-matched controls were used for Western blot and histologic analysis. Results: mTOR-related proteins and glucose transporter 1 (GLUT1) was upregulated at 1 month and downregulated in the following period up to 6 months. Diabetes-induced … olive clothing usWeb1 mai 2015 · Western blot assay demonstrated that the mTOR pathway in Y79 cells was blocked by rapamycin. Western blot and RT-PCR assay showed that Bmi-1 was downregulated in protein and mRNA level by rapamycin treatment. Further Western blot and RNA interference assays showed that rapamycin-mediated downregulation of Bmi-1 … olive clothing canadaWebWestern blot. H9C2 cells had been lysed by RIPA buffer (P0013B, Beyotime Biotech, Shanghai, China). Then the lysate was centrifuged at 12,000g for 15 min at 4 °C for collecting the supernatant. ... AMPK, mTOR, p-AMPK and p-mTOR by Western blot detection. (F) The correlation between BAX and LC3 II was obtained from Western blot … is alcohol the most abused drugWeb14 apr. 2024 · In this regard, the Western blotting method was employed to validate that the USP5 expression levels could be up-regulated after mTOR signaling pathway activation in H1299 and A549 cell lines. Rapamycin, an mTOR signaling pathway inhibitor, was used to identify that the expression of USP5 was regulated by the mTOR signaling pathway. olive clutch handbagsWebQuantitative Western blot analysis demonstrated that phenformin increased mTORC2 signaling and mTOR Ser 1261 and raptor Ser 792 phosphorylation (fig. S2B) in a … is alcohol toxic to dogsWebIn this study, the expression of DEPTOR, mTOR, RICTOR, RAPTOR and S6 kinases were investigated in SKOV-3 and PEO1 parental and the paclitaxel-resistant (TaxR) SKOV … olive clutch