NettetTwo-color flow cytometric analysis of CD206 (MMR) expression by GM-CSF-stimulated monocytes. Human peripheral blood mononuclear cells were cultured for 3 days with Recombinant Human GM-CSF (Cat. No. 550068). The cells were harvested, preincubated with Human BD Fc Block™ (Cat. No. 564219/564220), and stained with FITC Mouse … NettetFlow cytometric analysis of CD14 expression on human peripheral blood monocytes. Whole blood was stained with BD Horizon™ BV786 Mouse Anti-Human CD14 antibody (Cat. No. 563698/563699; solid line histogram) or with a BD Horizon™ BV786 Mouse IgG2a, κ Isotype Control (Cat. No. 563732; dashed line histogram).
A Protocol for the Comprehensive Flow Cytometric …
NettetEosinophils isolated from mouse blood and mouse lung tissue. Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator were used to isolate eosinophils from mouse blood and mouse lung. Isolation from blood resulted in highly enriched eosinophils, which were stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ for … Nettet13. sep. 2024 · Finally, to demonstrate blood cell recruitment, peritoneal infiltrates were immunophenotyped by flow cytometry. Washes from mice undergoing adhesion induction were collected and analyzed for Gr-1, CD11b, and F4/80 expression 18 … cycloplegics and mydriatics
CD11b immunophenotyping identifies inflammatory profiles in the mouse ...
Nettet3.2. Mat Inhibits CCl 4-Induced Hepatic Infiltration of Monocytes Subset. To elucidate the effects of Mat on infiltration of different monocyte subsets following liver injury, we further isolated NPCs from livers of CCl 4-challenged mice and characterized the different monocyte subset composition by flow cytometry analysis.As shown in Figure 2(a), … NettetHowever, a systematic flow cytometric approach to identify subsets of macrophages and dendritic cells (DCs) accurately and consistently in the normal mouse lung has not been described. Here we developed a panel of surface markers and an analysis strategy that accurately identify all known populations of macrophages and DCs, and their … Nettet17. jan. 2024 · To validate the array data, we determined expression of these receptors by flow cytometry in C-MOs from the blood and MAMPCs and MAMs from the lungs of mice with metastatic tumors. We confirmed that MAMPCs and MAMs in the metastatic lung expressed significantly higher levels of CD14, CD36, CD64 (Fcgr1), CD206 (Mrc1) and … cyclopithecus