WebNational Center for Biotechnology Information WebJan 6, 2024 · In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype …
Did you know?
WebDec 3, 2010 · Extensive mutagenesis combined with the atomic structures of the DNA packaging/terminase proteins gp16 and gp17 elucidated the ATPase and nuclease … WebApr 21, 2006 · Double-stranded DNA packaging in bacteriophages is driven by one of the most powerful force-generating molecular motors reported to date. The phage T4 motor is composed of the small terminase protein, gpl6 (18kDa), the large terminase protein, gp17 (70kDa), and the dodecameric portal protein gp20 (61kDa). gp16, which exists as an …
WebSep 6, 2013 · To study the assembly of the different structural components of the tail complex, we tested whether the 8-11-12 complex was able to incorporate gp17 protein in vitro.The extracts containing the 8-11-12 complexes and the extract derived from the separately cloned gp17 were incubated together at 30 °C, purified, and then analyzed for … WebMar 31, 2008 · Viral genomes are packaged into "procapsids" by powerful molecular motors. We report the crystal structure of the DNA packaging motor protein, gene product 17 (gp17), in bacteriophage T4. The structure consists of an N-terminal ATPase domain, which provides energy for compacting DNA, and a C-terminal nuclease domain, which …
WebFamily members such as protein 17 (gene product 17/gp17) found in Bacillus phage phi29, is involved in DNA replication and in pulling the phage DNA into the cell during the … WebGp17 found in other bacteriophages such as SPP1 (siphophage SPP1, a lytic Bacillus subtilis phage) has been identified as a tail completion protein adopting an alpha/beta fold, and found to be located at the interface between the head-to-tail connector and the tail of bacteriophage SPP1. Links ? Statistics ? Structure ? PubMed References
WebDec 3, 2010 · The bacteriophage T4 capsid is an elongated icosahedron, 120 nm long and 86 nm wide, and is built with three essential proteins; gp23*, which forms the hexagonal capsid lattice, gp24*, which forms pentamers at eleven of the twelve vertices, and gp20, which forms the unique dodecameric portal vertex through which DNA enters during …
WebJun 20, 2024 · Multiple methods were utilised to select or enrich for chimeric phages incorporating the K1F gp17 alone, including trxA, host-specificity, and CRISPR-Cas-based selection. Irrespective of the selection method, the above strategy yielded poorly reproducible phage propagation on the new host, indicating that the chimeric phage was … fish fertilizer bunningsWebFeb 16, 2024 · Like lambdoid N proteins, Gp17 has an arginine-rich motif (ARM), and mutations in its arginine codons inhibit its function. In infection assays using the mutant phage mEp021ΔGp17::Kan (where gp17 has been deleted), gene transcripts located downstream of transcription terminators were obtained only when Gp17 was expressed. fish fertilizationWebOct 23, 2007 · After injection of the microspheres carrying the prohead–gp17 complexes into the measurement chamber (an essentially infinite dilution), packaging was only initiated when the DNA was presented within ≈20 s. Similar behavior was also observed with the phage φ29 motor (J. Rickgauer, D.N.F., and D.E.S., unpublished data). fish fertilizer alaskaWebMar 9, 2024 · After recombination, this phage population contained primarily T7 acceptor phages with a small percentage of recombined phage … fish fertilizer wikipediaWebMar 31, 2008 · We report the crystal structure of the DNA packaging motor protein, gene product 17 (gp17), in bacteriophage T4. The structure consists of an N-terminal ATPase … fish festWebMar 7, 2008 · In phage T4, there is evidence that the large terminase protein, gene product 17 (gp17), assembles into a multisubunit motor and translocates DNA by an inchworm mechanism. gp17 consists of two domains; an N-terminal ATPase domain (amino acids 1-360) that powers translocation of DNA, and a C-terminal nuclease domain (amino acids … can a profit sharing plan be rolled to an iraWebpart of phage WO. One additional phage pseudogene, Wbm5055, is homologous to a conserved hypothetical protein gene found in WO prophages from wPip (WP1304), … fish fertilizer and pepper plants