WebApr 14, 2024 · Background In mouse, it was discovered that resveratrol (Res) enhanced osteoporosis (OP) by boosting osteogenesis. Besides, Res can also have an impact on MC3T3-E1 cells, which are crucial for the control of osteogenesis and thus increase osteogenesis. Although some articles have discovered that Res enhanced autophagy to … The green fluorescent protein and LC3 (GFP-LC3) fusion protein provides a useful indicator of autophagy initiation through the evaluation of LC3 dots or punctae. When autophagy occurs, GFP-LC3 foci redistribute from a diffuse pattern to a punctate cytoplasmic pattern (GFP-LC3 punctae) and the … See more The human liver cancer cell line HepG2 was obtained from the American Type Culture Collection (Rockville, Md., USA) and maintained at the Institute of Biochemistry and … See more To induce autophagy, HepG2 cells were treated with 130 μM galangin for 24 hours. For the co-immunoprecipitation experiments, HepG2 cells were treated with 130 μM galangin … See more Galangin (PubChem CID: 5281616) was purchased from Sigma–Aldrich and dissolved in dimethyl sulfoxide (DMSO) before addition to the cell cultures. The final concentration of … See more HepG2 cells were harvested by trypsinization, then washed twice with PBS, fixed with the buffer containing 3% glutaraldehyde and 0.1 M cacodylate and then re-fixed in osmium tetroxide. HepG2 cells were embedded … See more
Chemopreventive and Chemotherapeutic Effect of Propolis and Its ...
Web한의기술응용센터 WebJul 27, 2016 · Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma (HCC) cells, but the precise mechanism is not clear. In this study, we demonstrated that... shonky hall of fame
Galangin autophagy inducing flavonoid 548-83-4 - Sigma-Aldrich
WebSep 27, 2024 · Autophagy inhibitors were used to further probe the mechanism of GG-induced autophagy. We co-treated U87MG and U251 cells with GG and 3-methyladenine (3-MA; Selleck,TX, USA) or CQ, which block early and late phases of autophagy, respectively, and examined protein levels by western blotting. WebJul 27, 2016 · Abstract Galangin suppresses proliferation and induces apoptosis and autophagy in hepatocellular carcinoma (HCC) cells, but the precise mechanism is not … WebJan 29, 2024 · In this study, the protective mechanisms conferred by galangin were assessed with an emphasis on the induction of autophagy and regulation of the gut microbiota using a DSS-induced UC mouse model. 2. Materials and Methods 2.1. Chemicals Galangin (purity: ≥99%) was obtained from Biopurify Phytochemicals Ltd. (Chengdu, … shonky products