Web5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation,because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. WebBrdU Staining Protocol. Supplies · Ethanol 100% USP (highest quality) · FACS Staining Buffer (1XPBS w/ 3% calf serum, 0.05% sodium azide-filtered) —Dilute staining …
BrdU Monoclonal Antibody (BU20A), APC (17-5071-42)
WebFlow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Contact Details Facility Manager: Ferenc Livak, MD Phone: … WebFlow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Contact Details Facility Manager: Ferenc Livak, MD Phone: 240-760-7605 Email: [email protected] Address: Building 37, Rooms 6008 and 6011, NCI-BethesdaFacility Head: Subhadra Banerjee, PhD Phone: 240.760.7591 Email: … 鷹 ステッカー 車
BrDU Staining Protocol - Flow Cytometry
http://www.icms.qmul.ac.uk/flowcytometry/uses/cellcycleanalysis/bromodeoxyuridine/index.html WebThe BrdU solution has been shown to be stable for up to 4 months at 2 to 8°C, or it can be refrozen. Avoid multiple freeze-thaw cycles. DNase stock solution (1 mg/mL) may be refrozen once before it loses activity Data Sheets Safety Data Sheet User Guide/Manual 559619 Rev. 1 Please refer to Support Documents for Quality Certificates WebDec 13, 2010 · Actually the FACS plot is intended to show cell cycle state of cells within the BrdU incubation time. So, the cells entering S-phase will incorporate BrdU nucleotides into newly synthesized... 鷹 オイル