WebHan et al. survey ribosome collision sites across the transcriptome by sequencing disome footprints in humans and zebrafish and find that inefficient contexts for elongation, termination, and recycling of ribosomes contributes to the collision of ribosomes. They also uncover XBP1u as an endogenous target of ribosome-associated quality control. WebFeb 2, 2024 · The Disome-seq data generated in this study has been deposited into the GEO database under accession ID GSE201364. Source data are provided with this paper. Code availability
Reanalysis of ribosome profiling datasets reveals a function of ...
WebOnce the counts are saved under count_data folder, whole analysis, which can be configured in settings.R script can be called by./analysis.R OUTPUT_DIR VERSION. … WebDisome footprints were 57–63 nt long, consistent with ... This metagene analysis illustrated that the 50 ends of monosome footprints (Figure 1C, top) exhibited apeak 19 … sanar naturals collagen cream
Figures and data in Distinct elongation stalls during ... - eLife
WebSep 17, 2024 · Using a modified ribosome profiling protocol based on footprints from two closely packed ribosomes (disomes), we have mapped ribosomal collisions transcriptome-wide in mouse liver. ... Di_ZT0_1; ZT0, disome footprint, rep1: GSM3955376: Di_ZT0_1; ZT0, disome footprint, rep2: GSM3955377: Di_ZT2_1; ZT2, disome footprint, rep1 … WebJan 1, 2024 · Co-co assembly candidates are identified by a shift of the footprint density from monosome to disome fraction, or by a disome-over-monosome enrichment profile … Webmonosome footprints (Figure 1D). This 30-nt spacing between the two peaks perfectly fits one in-frame ribosome, suggesting that the 58-nt disome footprints were composed of two collided ribosomes of which the downstream one was paused. The sharp peak in Figure 1D also indicates that disome-seq detects ribosome collisions at codon resolution. sanar healthcare