WebMar 11, 2024 · To obtain CD34 + HSPCs, granulocyte colony-stimulating factor–mobilized mononuclear cells from a healthy donor were purchased from Key Biologics. CD34 + cells were purified with CliniMACS CD34 microbeads (Miltenyi Biotech, 130-017-051). At least 95% of purified cells were CD34 +. WebThe CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ …
CD14 positive cells accelerate hematopoietic stem cell …
WebFeb 8, 2000 · It was then incubated for 30 min at room temperature with 7.5 ml of murine anti-CD34 antibody (AC 101) conjugated to iron–dextran microbeads (AmCell). The product was then washed twice in PBS... WebWe studied whether superparamagnetic nanoparticles (CD34 microbeads), used clinically for specific magnetic sorting, can be used as a magnetic cell label for in vivo cell … focusrite solo thomann
Magnetic resonance tracking of human CD34+ progenitor cells
WebThe CliniMACS CD34 Reagent System is indicated for processing hematopoietic progenitor cells collected by apheresis (HPC, Apheresis) from an allogeneic, HLA-identical, sibling … WebThe CliniMACS® CD34 Reagent System is an FDA-approved method for selecting hematopoietic stem cells from donor apheresis, while passively depleting T cells that can … Webapheresis, and CD34+ cells were isolated by immunomagnetic separation using the CliniMACS affinity-based technology (CliniMACS CD34 MicroBeads; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation. Reagents, tubing sets, and buffers are purchased from Miltenyi Biotec. focusrite scarlett sound card